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International Journal of Botany
  Year: 2010 | Volume: 6 | Issue: 1 | Page No.: 1-10
DOI: 10.3923/ijb.2010.1.10
RAPD, ISSR and RFLP Analysis of Phylogenetic Relationships Among Congeneric Species (Anthemideae, Asteraceae) in Egypt
M.H. Abd El-Twab and F.A. Zahran

Abstract:
RAPD, ISSR and RFLP were used to study phylogenetic relationships among ten species belong to six genera (Asteraceae) in Egypt. Hundred and thirty one bands were resulted after 15 RAPD primers. The average of similarity coefficient was 0.0-0.286. The DNA of Matricaria recutita L., Achillea fragrantissima (Forssk.) Sch. Bip and Artemisia arborescens L., showed 60, 30 and 23 bands, respectively. Glebionis coronaria (L.) Cass. ex Spach, Art. judaica L., Cotula cinerea Del, Anacyclus monanthos (L.) Thell and Achillea santolina L., showed 7, 5, 3, 2 and 1 bands, respectively, while Cotula barbata DC. and Mat. aurea (Loefl.) Sch. Bip showed no bands. Eighteen bands were resulted after four ISSR primers. The average of similarity coefficient was 0.0-0.25. The DNA of Ach. fragrantissima, Mat. recutita, Art. arborances, Gle. coronaria and Ana. monanthos showed 6, 4, 4, 3 and 2 bands, respectively, while Cot. barbata, Cot. cinerea, Mat. aurea, Ach. santolina and Art. judaica showed no bands. Twenty six RFLP bands were detected after EcoR I and BamH I. The average of similarity coefficient was 0.0-0.5. The band products of the restricted DNA of Ach. fragrantissima, Art. arborescens, Art. judaica, Mat. aurea, Gle. coronaria, Cot. barbata and Cot. cinerea were 6, 4, 5, 4, 3, 2 and 2 bands, respectively, while Ach. santolina, Ana. monanthos and Mat. recutita showed no bands. Based on the present study the species were classified into Mat. recutita as out group; Ach. fragrantissima and Art. arborescens as related group and a group of seven species were closely genetical distance.
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How to cite this article:

M.H. Abd El-Twab and F.A. Zahran, 2010. RAPD, ISSR and RFLP Analysis of Phylogenetic Relationships Among Congeneric Species (Anthemideae, Asteraceae) in Egypt. International Journal of Botany, 6: 1-10.

DOI: 10.3923/ijb.2010.1.10

URL: https://scialert.net/abstract/?doi=ijb.2010.1.10

 
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