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International Journal of Botany

Year: 2007 | Volume: 3 | Issue: 4 | Page No.: 390-393
DOI: 10.3923/ijb.2007.390.393

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Authors


M.Z. Karim

Country: Japan

S. Yokota

Country: Japan

M.M. Rahman

Country: Japan

J. Eizawa

Country: Japan

Y. Saito

Country: Japan

M.A.K. Azad

Country: Japan

F. Ishiguri

Country: Japan

K. Iizuka

Country: Japan

N. Yoshizawa

Country: Japan

Keywords


  • root explant
  • adventitious shoot
  • Aralia elata
  • BT medium
  • PGR
Research Article

Efficient Adventitious Shoot Regeneration from Root Explants of Aralia elata Seem

M.Z. Karim, S. Yokota, M.M. Rahman, J. Eizawa, Y. Saito, M.A.K. Azad, F. Ishiguri, K. Iizuka and N. Yoshizawa
A highly reproducible in vitro shoot regeneration system in Aralia elata Seem. using root explants was developed in this investigation. Multiple shoots were induced in vitro directly from root explants through adventitious shoot bud regeneration. The ability of root explants to produce shoot buds depended on the supplementation of Plant Growth Regulators (PGRs). Maximum multiplication of shoots (18 shoots per explant) was achieved in a Broad-leaved Tree (BT) medium supplemented with 1.00 μM 6-benzyl aminopurine (BAP). The best and healthiest rooting was observed in a BT medium supplemented with 2.00 μM μ-naphthaleneacetic acid (NAA). Regenerated plants were successfully acclimatized under ex vitro condition.
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How to cite this article

M.Z. Karim, S. Yokota, M.M. Rahman, J. Eizawa, Y. Saito, M.A.K. Azad, F. Ishiguri, K. Iizuka and N. Yoshizawa, 2007. Efficient Adventitious Shoot Regeneration from Root Explants of Aralia elata Seem. International Journal of Botany, 3: 390-393.

DOI: 10.3923/ijb.2007.390.393

URL: https://scialert.net/abstract/?doi=ijb.2007.390.393

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