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International Journal of Botany
  Year: 2005 | Volume: 1 | Issue: 2 | Page No.: 175-182
DOI: 10.3923/ijb.2005.175.182
 
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Construction of a Dehydrin Gene Cassette for Drought Tolerance from Wild Origin for Wheat Transformation

Salwa Badr, Ahmed Bahieldin , Bassem Abdelgawad and Abdelfattah Badr

Abstract:
Construction of the dehydrin gene was made in a plant expression vector carrying the bar gene as a selectable marker in order to use in transforming wheat cultivar Giza 164. Plant transformation was made to the immature embryo-derived calli using biolistic bombardment. A tissue culture and transformation protocol were performed and bar gene was used as a selectable marker against the herbicide bialaphos. Five putative transgenic plants were successfully detected by leaf painting with the herbicide. Molecular analyses were done for these plantlets at the structural level; three gave positive results with PCR. The integration of the dehydrin gene into the genomic background of two transgenic plants was confirmed by Southern blotting.
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How to cite this article:

Salwa Badr, Ahmed Bahieldin , Bassem Abdelgawad and Abdelfattah Badr , 2005. Construction of a Dehydrin Gene Cassette for Drought Tolerance from Wild Origin for Wheat Transformation. International Journal of Botany, 1: 175-182.

DOI: 10.3923/ijb.2005.175.182

URL: https://scialert.net/abstract/?doi=ijb.2005.175.182

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