Micro-propagation of Damask Rose (Rosa damascena Mill.) cv. Almarah


	International Journal of Agricultural Research
	Year: 2013 \| Volume: 8 \| Issue: 4 \| Page No.: 172-177 DOI: 10.3923/ijar.2013.172.177

Micro-propagation of Damask Rose (Rosa damascena Mill.) cv. Almarah

T. Alsemaan

Abstract: Almarah is the most important cultivar of Damask rose in Syria. This study aimed to determine the best protocol for its in vitro micro-propagation. The experiments were conducted at Damascus University in Syria. Effects of different concentrations of benzyladenine BA (0, 0.5, 1 and 2 mg L^-1) and gibberellic acid GA3 (0, 1 and 2 mg L^-1) were evaluated on proliferation of the explants. To evaluate in vitro rooting, proliferated micro-shoots were cultured on a MS/2 medium supplemented with 3 g L^-1 activated charcoal and (0, 1, 2 and 3 mg L^-1) concentrations of Indol-3-Butyric Acid IBA. The experimental design of the all stages was a Complete Randomized Design. Means were analyzes with Duncan’s test at p≤0.05 using SPSS. BA increased the proliferation of the explants up to 2 mg L^-1. The interaction of BA with GA3 significantly increased proliferation rate of the explants. The highest number of proliferated explants was obtained in the presence of 2 and 2 mg L^-1 of GA3 and BA, respectively. The highest percentage of rooted explants was obtained in the presence of 3 mg L^-1 IBA in the rooting medium but the number and length of roots significantly increased in response to increasing IBA concentration in the medium up to 2 mg L^-1. Only 8% of the plantlets lost during hardening. For the first time in Syria a mass in vitro production protocol of Syrian Damask rose has been achieved. The interaction of BA (2 mg L^-1) with (GA3 2 mg L^-1) is highly recommended for proliferation rate of the explants while adding IBA (2 mg L^-1) to the MS/2 rooting medium is highly recommended for high quality in vitro rooting.

PDF Fulltext XML References Citation Report Citation

RELATED ARTICLES:

•	Genetic Diversity and Qualitative Variation of Rosa damascena in Syria

•	An Improved Micropropagation Protocol for Plumbago zeylanica L. An Important Medicinal Plant

•	In vitro Micropropagation using Corm Bud Explants: An Endangered Medicinal Plant of Gloriosa superba L.

•	In vitro Callus Induction and Shoot Multiplication from Nodal Explants and Leaves of Memecylon edule

How to cite this article:

T. Alsemaan , 2013. Micro-propagation of Damask Rose (Rosa damascena Mill.) cv. Almarah. International Journal of Agricultural Research, 8: 172-177.

DOI: 10.3923/ijar.2013.172.177

URL: https://scialert.net/abstract/?doi=ijar.2013.172.177

COMMENTS

14 February, 2015
mojgan arefi:
hi.
plz give me article for tissu culture of rosa damascena.
thanks.

REPLY

COMMENT ON THIS PAPER

Navigation


		Online First
		Current Issue
		Previous Issues
		Editorial Board
		Submit a Manuscript
		Guide to Authors
		Subscribe to E-alerts

Indexed In


		ASCI-Database
		Asian Digital Library
		Cambridge Scientific Abstract
		Chemical Abstract Services
		FSTA
		Google Scholar

Follow @scialert

Science Alert