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International Journal of Agricultural Research
  Year: 2012 | Volume: 7 | Issue: 3 | Page No.: 107-120
DOI: 10.3923/ijar.2012.107.120
 
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Discriminative Features of Some High and Low Molecular Weight Glutenin Genes in Different Wheat Species
M.N.M. Esmaail, E.A.A. Abd-Elhady, S.A. Edris, F.M. El Domyati, N.R. Abdelhamid, A.H.M. Hassan and A.A. El Seoudy

Abstract:
The present study was carried out to amplify glutenin genes to follow up any variations among different species of wheat genomes. Four designed specific primers of HMW-GS (HMW SX and HMW SY) and LMW-GS (LMW 7,8 and LMW S1) were used. In respect to the HMW-GS, the Subunit y gave higher polymorphism than subunit x, which failed to amplify gene patterns with diploid source of genome A and tetraploid species. While the polymorphism of subunit x appeared in variable size. In contrast, primers LMW 7, 8 and LMW S1 showed variations among the wheat species. The qualitative analysis of gene expression of HMW genes was studied by the SDS-PAGE, which was supported for the molecular studies and more informative approach to detect variations between and within species. Generally, the results of SDS-PAGE showed that, the absence or presence of GluA1 allele seemed to be responsible for clustering the cultivars and the species.
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How to cite this article:

M.N.M. Esmaail, E.A.A. Abd-Elhady, S.A. Edris, F.M. El Domyati, N.R. Abdelhamid, A.H.M. Hassan and A.A. El Seoudy, 2012. Discriminative Features of Some High and Low Molecular Weight Glutenin Genes in Different Wheat Species. International Journal of Agricultural Research, 7: 107-120.

DOI: 10.3923/ijar.2012.107.120

URL: https://scialert.net/abstract/?doi=ijar.2012.107.120

 
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