Submit Manuscript

International Journal of Agricultural Research

Year: 2011 | Volume: 6 | Issue: 5 | Page No.: 437-443
Facebook Twitter Digg Reddit Linkedin StumbleUpon E-mail

Article Trend

Total views 466

Search

Authors

V. Vijaya Geetha

P. Balamurugan

Keywords

Research Article

SDS PAGE Electrophoresis in Mustard Cultivars

V. Vijaya Geetha and P. Balamurugan
The main aim of the study was to discriminate the cultivars of mustard based on the nature of seed protein present in the seeds. Analysis of total soluble proteins (Tris-HCl Soluble) in seeds was done by Polyacrylamide Gel Electrophoresis (PAGE) method. Electrophoresis of seed proteins showed a total of 7 bands in Maya, GM-2, Varuna, PCR 7 and Pusa Bold whereas for rest of the genotypes some bands were absent. The critical bands for identification were the bands at Rf values 0.237, 0.327 and 0.667 which formed the 1st, 3rd and 7th band, respectively. The intensity of the band also varied among all the genotypes. Hence the present study obviously indicated the use of SDS page profile through electrophoresis for discrimination of mustard genotypes.
PDF Fulltext XML References Citation

Leave a Comment

Your email address will not be published. Required fields are marked *