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International Journal of Agricultural Research
  Year: 2009 | Volume: 4 | Issue: 8 | Page No.: 237-249
DOI: 10.3923/ijar.2009.237.249
 
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Identification and Characterization of HMW Glutenin Subunits and their Coding Sequences in Dwarfing Polish Wheat
Xing Fan, Zi-Jian Song, Hou-Yang Kang, Rui-Wu Yang and Yong-Hong Zhou

Abstract:
To evaluate High Molecular Weight (HMW) glutenin subunit comparison in Triticum turgidum sp. polonicum, HMW glutenin subunits of 22 accessions were analyzed using SDS-PAGE. The result showed that the variation in the number of HMW glutenin subunits in this taxon ranged from 1-3. A dwarfing accession of T. turgidum sp. polonicum (dwarfing polish wheat) from Xingjiang in China has two putative expressed HMW glutenin subunits. Three complete coding region sequences of HMW glutenin subunit genes were amplified from dwarfing polish wheat using PCR method. The PCR products were derived from 1Ax (1Ax-dp), 1Bx gene (1Bx-dp) and a silent 1Ay (1Ay-dp) gene. The complete ORF of x-type HMW glutenin genes, 1Ax-dp and 1Bx-dp, were 2508 and 2385 bp in size, encoding 836 and 795 amino acids, respectively. The silent 1Ay-dp gene contained an in-frame internal stop codon and was 1806 bp in size. Sequence comparison indicated that they had primary structure identical to the published HMW glutenin subunits. Phylogenetic analysis suggested that 1Ax-dp from dwarfing polish wheat was most closely related to 1Ax1 and 1Ax2* from Triticum aestivum, while 1Bx-dp from dwarfing polish wheat was most closely related to 1Bx7 from Triticum aestivum. Based on sequence comparison, SDS-PAGE and phylogenetic analysis, it can be deduced that 1Ax-dp and 1Bx-dp are additional alleles at the Glu-A1 and Glu-B1 loci, respectively.
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How to cite this article:

Xing Fan, Zi-Jian Song, Hou-Yang Kang, Rui-Wu Yang and Yong-Hong Zhou, 2009. Identification and Characterization of HMW Glutenin Subunits and their Coding Sequences in Dwarfing Polish Wheat. International Journal of Agricultural Research, 4: 237-249.

DOI: 10.3923/ijar.2009.237.249

URL: https://scialert.net/abstract/?doi=ijar.2009.237.249

 
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