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International Journal of Agricultural Research
  Year: 2006 | Volume: 1 | Issue: 1 | Page No.: 14-19
DOI: 10.3923/ijar.2006.14.19
 
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Adventitious Bud Induction and Plant Regeneration in Epicotyl Segments of Bakrai (Citrus reticulata BlancoxC. limetta Swing.)
M. Dejam , M. Khosh-Khui and A. Shekafandeh

Abstract:
Experiments were conducted to study the in vitro propagation of Bakrai using epicotyl explants. Explants were excised form in vitro grown seedlings, cultured on a basic MS medium supplemented with various concentrations of benzyladenine (BA) and naphthaleneacetic Acid (NAA). The highest percentage of explants producing normal adventitious shoots (83.3%) was obtained on a medium MS medium containing 2 mg L-1 BA. The maximum number of adventitious buds (20.33) produced in 4 mg L-1 BA, but most of these buds remained quiescent. Addition of 0.5 mg L-1 NAA increased shoot number and shoot length but higher NAA concentrations had an inhibitory effect on shoot regeneration, particularly in 1 mg L-1 BA concentration. The highest number of shoots (5.5) and longest shoots (9.68 mm) were obtained in concentrations of 2 and 1 mg L-1 BA plus 0.5 mg L-1 NAA, respectively. The shoots were rooted on a basal medium with half-strength MS salts and various concentrations of indolebutyric acid (IBA) (0.5-10 mg L-1 ). The highest percentage of rooting (91.67%) and the maximum number (1.71) and length (20.33 mm.) of roots were obtained with 10 mg L-1 IBA. The plantlets were transferred to pots containing an equal mixture (v/v) of sand and loamy soil and successfully acclimatized to natural environment with a survival rate of 80%.
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How to cite this article:

M. Dejam , M. Khosh-Khui and A. Shekafandeh , 2006. Adventitious Bud Induction and Plant Regeneration in Epicotyl Segments of Bakrai (Citrus reticulata BlancoxC. limetta Swing.). International Journal of Agricultural Research, 1: 14-19.

DOI: 10.3923/ijar.2006.14.19

URL: https://scialert.net/abstract/?doi=ijar.2006.14.19

 
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