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Current Research in Chemistry
  Year: 2021 | Volume: 13 | Issue: 1 | Page No.: 1-6
DOI: 10.3923/crc.2021.1.6
 
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Production Of 2-O-Alpha-D-Glucopyranosyl-L-Ascorbic Acid by Transglycosylation Using Dextransucrase From Leuconostoc mesenteroides

Mary Helen Palmuti Braga Vettori, Kate Cristina Blanco and Jonas Contiero

Abstract:
Background and Objective: Ascorbic acid is one of the antioxidants active in several biological systems, which are the main oxygenated components and free radicals used mainly in pharmaceutical applications. However, it is highly unstable at temperature and light, losing the beneficial effects when exposed during use. This study had as main objective to improve the instability of ascorbic acid, directing the application of the new compound to the use of the topic. Materials and Methods: For this, an enzymatic transglycosylation of ascorbic acid was performed using sucrose as a donor in the reaction. Transglycosylation was catalyzed by purified dextransucrase after production by a bacterial lineage of Leuconostoc mesenteroides FT045B and which catalyzes the acceptable reaction to the reaction capable of training the α-glycosides ascorbic acid. In this research, the transglycosylation reaction was conducted using the dextransucrase enzyme FT045B, used by the Leukonostoc mesenteroids FT045B and characterized by thin-layer chromatography and mass spectrometry. Results: The production of AA-2G is carried out enzymatically. The thin layer chromatography plate after eluting the sample under UV light revealed the compound AA-2G. The peaks of the mass spectrum of the sample represented specifics characteristics of AA-2G. Conclusion: From the microbial enzymatic transglycosylation reaction of ascorbic acid using sucrose was possible to synthesize AA-2G with different stability characteristics.
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How to cite this article:

Mary Helen Palmuti Braga Vettori, Kate Cristina Blanco and Jonas Contiero, 2021. Production Of 2-O-Alpha-D-Glucopyranosyl-L-Ascorbic Acid by Transglycosylation Using Dextransucrase From Leuconostoc mesenteroides. Current Research in Chemistry, 13: 1-6.

DOI: 10.3923/crc.2021.1.6

URL: https://scialert.net/abstract/?doi=crc.2021.1.6

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