Subscribe Now Subscribe Today
Science Alert
 
FOLLOW US:     Facebook     Twitter
Blue
   
Curve Top
Current Research in Bacteriology
  Year: 2016 | Volume: 9 | Issue: 1 | Page No.: 1-8
DOI: 10.3923/crb.2016.1.8
 
Facebook Twitter Digg Reddit Linkedin StumbleUpon E-mail
Protection of Bacillus subtilis Against Bacteriophage Attack
E.M. Marei and I. Elmaghraby

Abstract:
Attempts were made to protect Bacillus subtilis, which can be used as a biofertilizer against bacteriophage infection. A bacteriophage resistant mutant of Bacillus subtilis was successfully isolated. Inocula of B. subtilis in forms of free cells, alginate immobilized cells and phage resistant mutant were prepared. Under cultivated soil conditions, inoculation of wheat plants with either immobilized cells or phage resistant mutant of B. subtilis resulted in increase in numbers of spore forming bacteria in the rhizosphere soils and significantly increased plant height (cm), fresh and dry weigh (gram per plant) as compared to plants inoculated with the free cells of B. subtilis. Presence of bacteriophage, in soil cultivated with wheat plants inoculated with free cells of B. subtilis, markedly reduced number of spore forming bacteria in rhizosphere soil and significantly reduced plant height (cm), fresh and dry weight (gram per plant) as compared to plants inoculated with free cells of B. subtilis in absence of phage. In plants inoculated with immobilized cells or phage resistant mutant of B. subtilis, no significant effect for the presence of phage was detected. The phage susceptible cells of B. subtilis and the phage resistant mutant ones were examined by scanning electron microscopy. The scanning electron micrographs indicated that the isolated phage resistant mutant of B. subtilis exhibited slight difference in the dimension of the cells and number of the pili as compared to the susceptible cells.
PDF Fulltext XML References Citation Report Citation
How to cite this article:

E.M. Marei and I. Elmaghraby, 2016. Protection of Bacillus subtilis Against Bacteriophage Attack. Current Research in Bacteriology, 9: 1-8.

DOI: 10.3923/crb.2016.1.8

URL: https://scialert.net/abstract/?doi=crb.2016.1.8

 
COMMENT ON THIS PAPER
 
 
 

 

 
 
 
 
 
 
 
 
 

 
 
 
 
 
 
 

       

       

Curve Bottom