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Current Research in Bacteriology
  Year: 2014 | Volume: 7 | Issue: 1 | Page No.: 1-11
DOI: 10.3923/crb.2014.1.11
 
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Biofilm Formation by Methicillin Resistant Staphylococcus aureus and their Antibiotic Susceptibility Pattern: An in vitro Study

Neetu Peedikayil John and Sevanan Murugan

Abstract:
Methicillin resistant Staphylococcus aureus (MRSA) is associated with high morbidity and mortality rates because of the development of multi-drug resistance and their ability to form biofilms. The present study was conducted to detect the biofilm formation among MRSA isolates and to determine their antibiogram-resistogram profile. A total of 259 S. aureus isolates were collected from tertiary care hospitals in Coimbatore, South India. They were subjected to antibiotic susceptibility testing by Kirby Bauer technique and thereafter screened for biofilm formation by three methods viz., Congo Red Agar Method, Tube Method and Tissue Culture Plate method. Out of 259 S. aureus isolates, 209 (80.69%) were confirmed as MRSA and only 35 (16.76%) of the MRSA isolates were found to be strong biofilm producers by Tissue Culture Plate method on the basis of optical density values. The antibiotic resistance pattern of biofilm producing MRSA isolates were found to be highly variable with maximum resistance (100%) to ampicillin, ampicillin/sulfbactam, ofloxacin, tetracycline, ciprofloxacin, cotrimoxazole and 100% sensitivity observed towards vancomycin. The statistical analysis revealed that TCP method showed higher specificity when compared to other two methods. Thus, biofilm producers were found to be highly resistant to almost all the classes of antibiotics except vancomycin. According, to this study, vancomycin was the effective drug of choice for the treatment of biofilm producing MRSA infections and Tissue culture plate is the reliable method for detecting the biofilm formation.
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How to cite this article:

Neetu Peedikayil John and Sevanan Murugan, 2014. Biofilm Formation by Methicillin Resistant Staphylococcus aureus and their Antibiotic Susceptibility Pattern: An in vitro Study. Current Research in Bacteriology, 7: 1-11.

DOI: 10.3923/crb.2014.1.11

URL: https://scialert.net/abstract/?doi=crb.2014.1.11

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