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Current Research in Bacteriology
  Year: 2010 | Volume: 3 | Issue: 1 | Page No.: 42-45
DOI: 10.3923/crb.2010.42.45
In vitro Activity of Ampicillin Against β-Lactamase Producing Enterobacterial Species
A.O. Shakak and H.A. Saeed

Abstract:
This study was undertaken to determine the in vitro activity of ampicillin against β-lactamase producing enterobacterial species. The study was conducted during the period December 2006-August 2009. Two hundred patients were investigated. Clinical specimens collected were 38.5% ear swabs, 36.5% wound swabs and 25% urine specimens. Cultivation of these specimens on enriched and/or differential media yielded 189 bacterial isolates, of which 96 were enterobacterial species. Among the enterobacterial isolates 62.5% were β-lactamase producers as indicated by β-lactamase acidometric method. Ampicillin activity against β-lactamase producers was determined by modified Kirby-Bauer Disc Diffusion technique. The technique was judged and approved by the National Committee for Clinical Laboratory Standards (NCCLS). The results showed that positive β-Lactamase isolates were E. coli 21.6%, K. pneumoniae 15.5%, P. mirabilis 13.7% and P. vulgaris 0.9%. The result indicated that only 8.6% of the β-lactamase producers were sensitive to ampicillin while 91.4% were resistant. It has been concluded that further studies are needed to validate the usefulness of ampicillin in treatment of enterobacterial infections in Sudanese patients.
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How to cite this article:

A.O. Shakak and H.A. Saeed, 2010. In vitro Activity of Ampicillin Against β-Lactamase Producing Enterobacterial Species. Current Research in Bacteriology, 3: 42-45.

DOI: 10.3923/crb.2010.42.45

URL: https://scialert.net/abstract/?doi=crb.2010.42.45

 
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