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Bacteriology Journal
  Year: 2018 | Volume: 8 | Issue: 1 | Page No.: 1-11
DOI: 10.3923/bj.2018.1.11
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Antimicrobial Activity of Essential Oil Against Rhizobium (Agrobacterium) vitis Using Agar Well and Disc Diffusion Method

Yesim Er , Nur Sivri and Mustafa Mirik

Background and Objective: Essential oils have played many important roles in Plant Protection. The aim of this study was to determine antimicrobial effects of some essential oils against mixture of seven different Rhizobium vitis isolates. Materials and Methods: Typical crown galls were collected from vineyards in the main grape-growing regions in Thrace region. Rhizobium spp., were isolated and streaked on RS and PDA medium. After incubation for 48 h at 28°C, Rhizobium colonies resembling A. vitis were selected and purified three times on PDA. Typical A. vitis colonies were streaked and maintained on PDA. After bacterial isolates were tested for colonization morphology and pathogenicity, essential oils were examined for six different concentration to determine antibacterial activity and select suitable method according to disc diffusion and well diffusion methods. The experiments were attempted with five replications, with positive and negative controls. Results: As a result, it has been observed that the disk diffusion method has a larger diameter and a more visible zone. According to inhibition zone formation, it has been found that John's wort , thyme and ginger essential oils, respectively are the most effective among the other essential oils and doses tested in disk diffusion and well diffusion methods. The highest antibacterial activity was observed in john's wort at 30 mg mL–1 concentration, while black cumin essential oil at 5 mg mL–1 concentration was the lowest antibacterial activity against A. vitis isolates. Component analysis of antibacterial essential oils were performed by GC/MS. The major volatile compounds in the John's wort, thyme and ginger essential oil were found to be camphor (20.67%), thymol (22.9%) and benzyl alcohol (43.07%), respectively. Conclusion: So, John's wort EO showed very high antibacterial activity and disc diffusion method showed more bacterial inhibition.
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How to cite this article:

Yesim Er, Nur Sivri and Mustafa Mirik, 2018. Antimicrobial Activity of Essential Oil Against Rhizobium (Agrobacterium) vitis Using Agar Well and Disc Diffusion Method. Bacteriology Journal, 8: 1-11.

DOI: 10.3923/bj.2018.1.11






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