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Bacteriology Journal
  Year: 2014 | Volume: 4 | Issue: 1 | Page No.: 1-11
DOI: 10.3923/bj.2014.1.11
Molecular Characterization of Marine Streptomyces enissocaesilis Capable of L-asparaginase Production
B. Sirisha, R. Haritha, Y.S.Y.V. Jagan Mohan, A. Swathi and T. Ramana

Abstract:
Actinomycete strain producing L-Asparaginase was isolated from a marine sediment sample from Bay of Bengal collected near Cudallore of Tamil Nadu state, India. Morphological and physiological characteristics as well as chemotaxonomic features of strain AUBT-1404 were congruent with the description of the genus Streptomyces. The isolate, AUBT-1404, was identified by the polyphasic approach. Phylogenetic analysis based on 16S rRNA gene sequences showed that the strain AUBT-1404 belonged to the genus Streptomyces, with the highest similarity to Streptomyces enissocaesilis NRRL B-16365T (99.86%). The isolate had cell-wall type I (LL-diaminopimelic acid and no diagnostic whole-cell sugars) and the diagnostic phospholipids were phosphatidylethanolamine and phosphatidylcholine. The predominant menaquinone was MK-9 (H4). The G+C content of the genomic DNA was 76.2 mol%. On the basis of polyphasic evidence, strain AUBT-1404 was recognized as a new strain of Streptomyces enissocaesilis.
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How to cite this article:

B. Sirisha, R. Haritha, Y.S.Y.V. Jagan Mohan, A. Swathi and T. Ramana, 2014. Molecular Characterization of Marine Streptomyces enissocaesilis Capable of L-asparaginase Production. Bacteriology Journal, 4: 1-11.

DOI: 10.3923/bj.2014.1.11

URL: https://scialert.net/abstract/?doi=bj.2014.1.11

 
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