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Bacteriology Journal

Year: 2012 | Volume: 2 | Issue: 4 | Page No.: 79-89
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Authors

Olujimi Dada

Mohd. Sahaid Kalil

Wan Mohtar Wan Yusoff

Keywords

Research Article

Effects of Inoculum and Substrate Concentrations in Anaerobic Fermentation of Treated Rice Bran to Acetone, Butanol and Ethanol

Olujimi Dada, Mohd. Sahaid Kalil and Wan Mohtar Wan Yusoff
The availability of ricebran; one of the by products of the rice-milling industry in cheap and large quantity, is utilised in this work for the production of renewable fuel through fermentation process. Treated ricebran, a lignocellulosic material considered as wastes from the rice milling industry was fermented anaerobically in a batch process using Clostridium saccharoperbutylacetonicum N1-4 as the inoculum at initial pH of 6±0.2 and temperature of 30°C for the determination of the most suitable initial concentration of ricebran and the inoculum concentration that will produce the maximum amount of products. Different ricebran initial concentrations, 10, 20, 50, 100 and 120 g L-1 were fermented using inoculum concentration of 1.5 g L-1. Effect of Inoculum concentration was also investigated by using inoculum concentrations of 0.5, 1.0, 1.5, 2.0 and 2.5 g L-1 for fermentation of ricebran hydrolysates at fixed substrate concentration and volume. Increment in substrate concentration had a significantly positive effect (p<0.05) on yield and productivity of acetone, butanol and ethanol up till concentration of 100 g L-1 after which further increment led to reduction in the yield and productivity. In addition, increment in inoculum concentration also had a significantly positive effect (p<0.05) on yield and productivity of the fermentation products up till concentration of 1.5 g L-1 after which further increment in inoculum concentration led to decrease in the yield and productivity. The highest products’(acetone, butanol and ethanol) yield and productivity; 0.39 g g-1 and 0.079 g L-1 h-1, respectively, were obtained at the initial ricebran concentration of 100 g L-1. In addition, the highest products’ (acetone, butanol and ethanol) yield and productivity; 0.39 g g-1 and 0.095 g L-1 h-1, respectively were obtained at an inoculum concentration of 1.5 g L-1. Higher inoculum concentration resulted in lower yield and productivity of acetone, butanol and ethanol. The best inoculum concentration and substrate concentration for production of acetone, butanol and ethanol from treated ricebran are 1.5 and 100 g L-1, respectively.
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How to cite this article

Olujimi Dada, Mohd. Sahaid Kalil and Wan Mohtar Wan Yusoff, 2012. Effects of Inoculum and Substrate Concentrations in Anaerobic Fermentation of Treated Rice Bran to Acetone, Butanol and Ethanol. Bacteriology Journal, 2: 79-89.

URL: https://scialert.net/abstract/?doi=bj.2012.79.89

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