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Biotechnology
  Year: 2021 | Volume: 20 | Issue: 1 | Page No.: 8-14
DOI: 10.3923/biotech.2021.8.14
 
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Enhancement of the Growth and Antimicrobial Activity of Pediococcus pentosaceus Strain 2397 against Staphylococcus aureus

Usman Pato , Yusmarini Yusuf, Shanti Fitriani, Tartila , Rahma Yeni, Fani Fadillah and Latifa Husnanini

Abstract:
Background and Objective: Bacteriocin, a peptide produced by Lactic Acid Bacteria (LAB) widely used as a natural and safe preservative in food products. This study aimed to determine the optimal incubation conditions and medium contents to increase the growth and antimicrobial activity of Pediococcus pentosaceus strain 2397 against Staphylococcus aureus. Materials and Methods: Pediococcus pentosaceus strain 2397 was used to study the antimicrobial activity against S. aureus FNCC-15. The cell-free supernatant and bacteriocin's antimicrobial activity from strain 2397 was carried out by the well diffusion method. Results: The results showed that the incubation time, the pH of the growth medium, the concentration of starter, the addition of various nitrogen and carbon sources affected the growth and antimicrobial activity of Pediococcus pentosaceus strain 2397. Optimal conditions for growth and increased antimicrobial activity of strain 2397 against S. aureus were obtained at 24 hrs incubation time, starter concentration 5.0%, medium pH 6, addition of N sources and C sources. Conclusion: Incubation conditions and medium composition determined the growth and antimicrobial activity of P. pentosaceus strain 2397 against S. aureus.
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How to cite this article:

Usman Pato, Yusmarini Yusuf, Shanti Fitriani, Tartila , Rahma Yeni, Fani Fadillah and Latifa Husnanini, 2021. Enhancement of the Growth and Antimicrobial Activity of Pediococcus pentosaceus Strain 2397 against Staphylococcus aureus. Biotechnology, 20: 8-14.

DOI: 10.3923/biotech.2021.8.14

URL: https://scialert.net/abstract/?doi=biotech.2021.8.14

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