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Biotechnology
  Year: 2021 | Volume: 20 | Issue: 1 | Page No.: 22-30
DOI: 10.3923/biotech.2021.22.30
 
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Amplification and Sequence Analysis of Indole-3-Pyruvic Acid (IPyA) Pathway Related Genes from Bacillus spp.

Karen B. Alviar , Krizzia Mae R. Lumangaya, Joan Christine O. Adajar and Mannix S. Pedro

Abstract:
Background and Objective: Several species of Bacillus are plant growth-promoting rhizobacteria that can produce the auxin phytohormone Indole-3 acetic acid (IAA) which regulates plant growth and development. This study aimed to amplify key genes, patB and dhaS, two of the three component enzymes found in the IPyA biosynthetic pathway for IAA synthesis and conduct 16S rRNA sequence analysis of two Bacillus isolates from Fermented Plant Juice (FPJ) extracted from Madre de Cacao, Gliricidia sepium. Materials and Methods: Genomic DNA extraction was performed using the CTAB method followed by the amplification of IPyA pathway genes through a polymerase chain reaction. Sequence similarity search was performed using NCBI BLASTn and the protein structure was predicted using Phyre2. Results: The dhaS gene from isolate 1 (B. amyloliquefaciens) had a top nucleotide BLAST hit with B. velezensis strain FJAT-45028 and a translated BLAST hit with an aldehyde dehydrogenase protein. The resulting rank three models in protein structure prediction revealed dhaS which encodes for an enzyme responsible for the conversion of indole-3-acetaldehyde (IAAld) to IAA. Furthermore, phylogenetic trees generated using the Maximum likelihood method revealed the relationship of each isolate with its top five BLAST hits. Conclusion: These amplified IAA-related genes may be further utilized in optimizing phytohormone production that has the potential to improve the capability of Bacillus isolates as biofertilizers.
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How to cite this article:

Karen B. Alviar, Krizzia Mae R. Lumangaya, Joan Christine O. Adajar and Mannix S. Pedro, 2021. Amplification and Sequence Analysis of Indole-3-Pyruvic Acid (IPyA) Pathway Related Genes from Bacillus spp.. Biotechnology, 20: 22-30.

DOI: 10.3923/biotech.2021.22.30

URL: https://scialert.net/abstract/?doi=biotech.2021.22.30

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