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  Year: 2021 | Volume: 20 | Issue: 1 | Page No.: 15-21
DOI: 10.3923/biotech.2021.15.21
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Characterization of Phosphate Solubilising Bacteria Isolated from Rhizosphere Soils of Piper nigrum L.

Ashritha , B. Raghavendra Rao , M. Ramya Rai, P. Nagaraj and P. Visweswara Rao

Background and Objective: Phosphorus is an important plant nutrient next to Nitrogen. The amount of phosphorus available for plant growth in the soil is very less due to its insoluble nature. In this study, isolation and evaluation of phosphate solubilising bacteria from the rhizosphere soils of Piper nigrum L. grown in two pepper growing locations of Karnataka state of India was carried out. Materials and Methods: Rhizosphere soil samples collected from two locations were subjected to serial dilution and plated on Sperber’s medium. These isolates were subjected to morphological and biochemical tests evaluation for plant growth promoting property and release of water soluble phosphorus. All experiments were performed in triplicates and data are expressed as Mean±standard deviation. Results: A total of eighteen phosphate solubilising bacterial isolates were isolated from the pepper rhizosphere soils samples from two different locations of Karnataka state of India. Isolate T6 released maximum water soluble phosphorus of 82 μg mL1 and compared to un inoculated control, all the treatments involving inoculation of Phosphate solubilising bacteria showed higher plant height. Among all the isolates, six isolates showed positive and twelve isolates showed negative results for gram reaction. All the isolates were positive for catalase and oxidase test and all were negative for urease production and lipase test. All the isolates were rod shaped. Conclusion: Phosphate solubilising bacterial isolates isolated from rhizosphere of P. nigrum L. exhibited solubilisation of insoluble phosphorus and also plant growth promotional property. They can be used to improve the phosphorus nutrition and growth of black pepper.
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How to cite this article:

Ashritha , B. Raghavendra Rao, M. Ramya Rai, P. Nagaraj and P. Visweswara Rao, 2021. Characterization of Phosphate Solubilising Bacteria Isolated from Rhizosphere Soils of Piper nigrum L.. Biotechnology, 20: 15-21.

DOI: 10.3923/biotech.2021.15.21






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