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  Year: 2021 | Volume: 20 | Issue: 1 | Page No.: 1-7
DOI: 10.3923/biotech.2021.1.7
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Extender and Cryoprotectant Assessment to Maximize the Competence of Cryostorage in Glossogobius giuris (Hamilton-buchanan) Spermatozoa

Leena Grace Beslin

Background and Objective: The species which are found only in fewer numbers is believed to be endangered and the conservation of these rare organisms turns out to be highly imperative. The present study elucidates the conservation of male gametes of Glossogobius giuris through the cryopreservation technique. Materials and Methods: The milt from matured males was collected and processed by the addition of two diluents such as tris glycerol and citrate glycerol along with additional sugars namely glucose, fructose and mannitol. Then the spermatozoa with cryoprotectants were processed and stored in liquid nitrogen for a period of 300 days. The liquid nitrogen protects the spermatozoa due to its deep freezing nature at -196°C. Results: The survivability of tris glycerol glucose combination ranged from 99±3.2-96.7±3.9% during the storage period. In tris glycerol fructose combinations, the motility range was 99.1±1.9 and 95.9±0.9%. The observations of motility and fertility tests showed that the tris glucose-glycerol and fructose-glycerol combinations proved to be superior cryoprotectants. Survivability of mannitol with citrate glycerol was 75±1.2 and 65±3.7%. In the current study this proved the least motility score. Conclusion: From this study it was suggested that glycerol glucose and fructose in tris combinations gave maximum protection to the finfish Glossogobius giuris sperms. The buffer citrate glycerol also performed well next to tris glycerol. These were documented to be non-toxic to fish milt.
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How to cite this article:

Leena Grace Beslin , 2021. Extender and Cryoprotectant Assessment to Maximize the Competence of Cryostorage in Glossogobius giuris (Hamilton-buchanan) Spermatozoa. Biotechnology, 20: 1-7.

DOI: 10.3923/biotech.2021.1.7






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