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Biotechnology

Year: 2019 | Volume: 18 | Issue: 2 | Page No.: 64-76
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Authors

Chitta Ranjan Deb

Chitta Ranjan Deb

Philip Kadunlung Gangmei

Keywords

Research Article

In vitro Propagation of Kiwi from Nodal Segments and Development, a New Rooting Technique of Micro Shoots

Chitta Ranjan Deb Chitta Ranjan Deb's LiveDNA and Philip Kadunlung Gangmei
Background and Objective: Actinidia deliciosa (A. Chev.) popularly known as Kiwi fruit is an important horticultural plant grown in different parts of the world. Common practice of propagation of the species is through stem cutting but due to poor rooting of stem cuttings limits the production of clonal planting materials . Present study was aimed at development of in vitro propagation protocol of the species for production of clonal planting materials. Materials and Methods: A successful attempt was made to develop an efficient in vitro propagation protocol using in vivo source nodal segments, an efficient rooting technique and primary hardening using some low cost substrata as agar alternative. Results: About 7 week old nodal segments of in vivo source collected during November were cultured on MS medium fortified with sucrose (3%, w/v), Polyvinylpyrrolidone (300 mg L–1), benzyl adenine (BA) (6 μM), where within 6 days of culture morphogenic response initiated followed by average 4.2 shoot buds formation in 75% cultured nodal segments. The unidonal nodal segments from the resultant shoot buds on initiation medium proliferated and developed multiple micro shoots on MS medium enriched with sucrose (3%) and BA (3 μM) where as many as 6.2 micro shoots developed in 58.3% culture per cycle. The well developed foliated and defoliated micro shoots (4-5 cm long) were rooted on nutrient medium containing indole butyric acid (9 μM) where 50 and 56.3% rooting response registered, respectively accompanied by 5 and 7 numbers of roots/shoot. The rooted plantlets were hardened on 1/4th strength liquid medium containing sucrose (1%) and chopped coconut coir for 7-8 weeks followed by transferred to potting mix where ~82% transplants survived. Conclusion: Outcome of the present work resulted a new route for efficient and low cost propagation protocol of Kiwi fruit.
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