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Biotechnology
  Year: 2018 | Volume: 17 | Issue: 2 | Page No.: 95-103
DOI: 10.3923/biotech.2018.95.103
Oxalic Acid as the Main Molecule Produced by Trichoderma asperellum MG323528 Fermented on Corn Stover Based Medium
Abdulaziz Abdulrahman Al-Askar, WesamEldin Ismail Ali Saber, Khalid Mohamed Ghoneem and Younes Mohamed Rashad

Abstract:
Background and Objective: Organic acids have several pharmaceutical, food, agricultural and medical applications. Corn stover represents a serious environmental problem. The present study investigated the bio processing of such readily available low-cost biomass with microorganism into valuable organic acids that expected to neutralize the negative impact on the environment and minimize the production costs. Materials and Methods: A novel cellulolytic Trichoderma asperellum MG323528 was selected as a new corn stover decomposer that could transform it into various bio-products. The fungus was incorporated in corn stover-based medium for the production of organic acids. The Box-Behnken experimental design was applied to maximize the total organic acids production especially oxalic acid. Results: The optimum composition of solid-state fermentation medium was found to contain17.83 mg P from rock phosphate, 5.61 mg N from (NH4)2SO4 and 9.84 mg MgSO4•7H2O per 1 g of corn stover, yielding a total of 209.11±1.20 mmol organic acids. According to the HPLC screening, the main organic acids detected in the fermented corn stover was oxalic acid, representing about 78% of the total organic acids, in addition to minor amounts of citric, formic, salicylic and ascorbic acids. Conclusion: This kind of homo-fermentation could be considered for large-scale production of oxalic acid on an economic medium of CS using the promising T. asperellum MG323528 strain.
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How to cite this article:

Abdulaziz Abdulrahman Al-Askar, WesamEldin Ismail Ali Saber, Khalid Mohamed Ghoneem and Younes Mohamed Rashad, 2018. Oxalic Acid as the Main Molecule Produced by Trichoderma asperellum MG323528 Fermented on Corn Stover Based Medium. Biotechnology, 17: 95-103.

DOI: 10.3923/biotech.2018.95.103

URL: https://scialert.net/abstract/?doi=biotech.2018.95.103

 
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