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Biotechnology
  Year: 2018 | Volume: 17 | Issue: 3 | Page No.: 142-150
DOI: 10.3923/biotech.2018.142.150
Molecular Characterization of Two New Mycoplasma Species Isolated from Chickens in Saudi Arabia
Magdy Hassan Yassin, Alaa Ahmed Mohamed, Mohamed Mahmoud Hassan , Ahmed Abd El-Aziz Baiomy and Amena Maher Ibrahim

Abstract:
Background and Objective: Avian Mycoplasma infections pose a problem in poultry breeding. The main objective in this study was to isolate and identify new endemic Mycoplasma species that cause poultry diseases in Taif, Saudi Arabia by using both traditional microbiological methods and modern molecular methods. Materials and Methods: One hundred forty lung tissue samples from native chickens were collected from a Taif poultry abattoir and subjected to culture isolation, pathological and molecular characterization. Results: Sixteen isolates (11.4%) were identified as Mycoplasma species based on their cellular, morphological and biochemical characteristics. Furthermore, Mycoplasma isolates were identified by 16S rRNA gene sequencing; seven Mycoplasma isolates were identified as Mycoplasma gallinaceum, which infects turkeys. Only one of these isolates was identified as Mycoplasma fermentans, which infects human and eight isolates were unknown Mycoplasma sp. They may be trans located between farmers and chickens or between turkeys and chickens in the same farm. This is the first report of isolation M. gallinaceum from native chickens in the Taif region, Saudi Arabia. Conclusion: These results confirmed that polymerase chain reaction could be used to rapidly diagnose and identify Mycoplasma and is sensitive to molecular detection. These obtained isolates could be applied in control strategies and vaccine development in Saudi Arabia.
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How to cite this article:

Magdy Hassan Yassin, Alaa Ahmed Mohamed, Mohamed Mahmoud Hassan, Ahmed Abd El-Aziz Baiomy and Amena Maher Ibrahim, 2018. Molecular Characterization of Two New Mycoplasma Species Isolated from Chickens in Saudi Arabia. Biotechnology, 17: 142-150.

DOI: 10.3923/biotech.2018.142.150

URL: https://scialert.net/abstract/?doi=biotech.2018.142.150

 
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