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Biotechnology
  Year: 2016 | Volume: 15 | Issue: 3-4 | Page No.: 86-95
DOI: 10.3923/biotech.2016.86.95
 
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Separation and Purification of Polyphenols from Pericarpium Granati Using Macroporous Resins and Evaluation of its Anti-Streptococcus mutans Activity in vitro
Xudong Jiang , Weihao Yang, Chuanjian Zhou, Kailing Lu and Cuiwu Lin

Abstract:
Background: The optimal parameters of separation and purification of polyphenols from Pericarpium Granati were determined and the antibacterial activities against S. mutans in vitro were investigated. Methodology: Through comparing the static adsorption/desorption properties of five kinds of tested macroporous resins for polyphenols, HPD-450 resin was selected to investigate optimal purification parameters by dynamic curve experiments. The optimal purification conditions as follows: 9.0 BV of feeding solution at concentration of 3.2 mg mL–1 through the column at 3.0 BV h–1, then for desorption 60% ethanol-aqueous 3.5 BV at 2.0 BV h–1 eluted. The content of polyphenols was increased from 34.9±1.6% to 71.1±3.1% with a recovery yield of 85.5±3.7% according to the optimal parameters. Results: The present study showed that the MIC of purity of polyphenols was 6.25 mg mL–1. The acidogenicity of S. mutans can be significantly inhibited when the concentration was up to or higher than 1.56 mg mL–1 and the adherence of S. mutans can be significantly inhibited when the concentration was up to or higher than 3.13 mg mL–1 as well. Conclusion: The results demonstrated that polyphenols from Pericarpium Granati have significant activity against S. mutans and it is a good candidate for further development as an oral care agent for preventing dental caries.
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How to cite this article:

Xudong Jiang, Weihao Yang, Chuanjian Zhou, Kailing Lu and Cuiwu Lin, 2016. Separation and Purification of Polyphenols from Pericarpium Granati Using Macroporous Resins and Evaluation of its Anti-Streptococcus mutans Activity in vitro. Biotechnology, 15: 86-95.

DOI: 10.3923/biotech.2016.86.95

URL: https://scialert.net/abstract/?doi=biotech.2016.86.95

 
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