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Biotechnology
  Year: 2015 | Volume: 14 | Issue: 5 | Page No.: 248-253
DOI: 10.3923/biotech.2015.248.253
 
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Partial Purification and Characterization of Cellulase from the Haemolymph of the African Giant Land Snail (Archachatina marginata)
Omolola E. Omotosho, A.C. Omonhinmin, S.O. Rotimi, E.E.J. Iweala, O.A. Rotimi and Ayoade Femi

Abstract:
The aim of this study is to partially purify and characterize the cellulase extracted from the snail haemolymph of the African giant land snail of the species, Archachatina marginata. The protein concentration of the haemolymph was found to be 168 mg mL–1 and the specific activity of the crude cellulase was found to be 0.007 U mg–1 protein using Carboxymethyl-cellulose (CMC) as the substrate. The partial purification on Sephadex G-200 produced two activity peaks. The molecular weights of the two cellulase fractions were estimated to be 112,202 and 67,000. The Michealis Menten constant (km) for cellulase was 4.3 and 2.8 mg mL–1 for the higher and lower molecular weight fractions, respectively. The multiplicity of cellulase complexes indicated that the haemolymph of A. marginata may convert derived cellulose in foodstuffs such as garlic, mushrooms and domestic wastes into sources of fuel. It is suggested that the enzyme can be used to produce value-added products, such as ethanol, citric acid, amino acids and vitamins including primary metabolites from cellulosic wastes.
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How to cite this article:

Omolola E. Omotosho, A.C. Omonhinmin, S.O. Rotimi, E.E.J. Iweala, O.A. Rotimi and Ayoade Femi, 2015. Partial Purification and Characterization of Cellulase from the Haemolymph of the African Giant Land Snail (Archachatina marginata). Biotechnology, 14: 248-253.

DOI: 10.3923/biotech.2015.248.253

URL: https://scialert.net/abstract/?doi=biotech.2015.248.253

 
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