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  Year: 2015 | Volume: 14 | Issue: 3 | Page No.: 136-141
DOI: 10.3923/biotech.2015.136.141
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Genotyping of Growth Hormone Gene in Egyptian Small Ruminant Breeds
Othman E. Othman, Sally S. Alam, Heba A.M. Abd El-Kader and Omaima M. Abd-El-Moneim

Small ruminants are considered as one of the major sources of meat and milk production in Egypt. Identification of the genes underlying livestock production traits leads to more efficient breeding programs and it is a promising way to improve production traits of farm animals. Growth hormone is a polypeptide hormone which is the major regulator of the metabolic procedures of growth and development and it is encoded by GH gene. In this study, we aimed to detect the genetic polymorphism of GH gene in major Egyptian sheep and goat breeds using PCR-RFLP and identify the single nucleotide polymorphism between different genotypes detected in these breeds. The primers used in this study flanked a 422 bp fragment from exons 2 and 3 of GH gene in sheep and goat. These PCR amplified fragments were digested with HaeIII endonuclease and the results showed the presence of two genotypes; GG and AG with the absence of AA genotype in 149 tested animals for this gene. The total frequencies were 43.56 and 56.44% for GG and AG genotypes, respectively in 101 tested sheep animals whereas in goat animals, the total frequencies were 12.5 and 87.5% for GG and AG genotypes, respectively in 48 tested goat animals. The sequence analysis of purified PCR products representing these two detected genotypes declared the presence of a SNP (G→A) at position 55 in the amplified fragment which is responsible for the destruction of the restriction site GG^CC and consequently the presence of two different alleles G and A which were named in this work according to the detected SNP. The nucleotide sequences of sheep GH alleles G and A as well as goat GH alleles G and A were submitted to nucleotide sequences database NCBI/Bankit/GenBank and have accession numbers: KP120857, KP120858, KP120859 and KP120860, respectively. In conclusion, production improvements can be achieved by using new genetic technology for better selection of heritable traits through marker-assisted selection. Due to the reported association between genotype possess A/G nucleotide with different growth trait parameters, we recommend to increase this heterozygous genotype in native small ruminant breeds through the selection of animals have AG genotype of GH gene and enter them in breeding programs of Egyptian small ruminants as a way to increase their production traits.
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How to cite this article:

Othman E. Othman, Sally S. Alam, Heba A.M. Abd El-Kader and Omaima M. Abd-El-Moneim, 2015. Genotyping of Growth Hormone Gene in Egyptian Small Ruminant Breeds. Biotechnology, 14: 136-141.

DOI: 10.3923/biotech.2015.136.141








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