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Biotechnology
  Year: 2013 | Volume: 12 | Issue: 4 | Page No.: 183-188
DOI: 10.3923/biotech.2013.183.188
 
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Probing the Single Nucleotide Polymorphism (Snp) of Swine PPAR Delta Gene

W.Y. Liu

Abstract:
Recently, it was reported that PPAR delta (PPARδ, alias PPARβ) serves as a key nuclear transcription factor regulating the expression of myriad genes in placent nutrient absorption and energy metabolism (lipid and glucose metabolism) which suggested it might be vital in placental development and pregnancy. In order to investigate and identify potential genetic markers in swine breeding, we designed two pairs of primers for Single Strand Conformation Polymorphism (SSCP) analysis based on the complete mRNA coding sequences of PPARδ gene and its 5’- flanking region. The sequencing result showed the amplification product of a pair of primers was polymorphic and one mutation C→T was identified in the second intron of PPARδ gene. Before the study, genetic polymorphisms of swine PPARδ gene were rarely reported. The present study provided useful information for future research in relevant medicinal genetics and animal breeding.
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How to cite this article:

W.Y. Liu , 2013. Probing the Single Nucleotide Polymorphism (Snp) of Swine PPAR Delta Gene. Biotechnology, 12: 183-188.

DOI: 10.3923/biotech.2013.183.188

URL: https://scialert.net/abstract/?doi=biotech.2013.183.188

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