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Biotechnology

Year: 2011 | Volume: 10 | Issue: 6 | Page No.: 546-550
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Authors

Afaque Quraishi

Shailesh Kumar Jadhav

Sheetal Gupta

Keywords

Research Article

In vitro Clonal Propagation of Cassia tora L. (Coffee Pod): A Medicinal Plant

Afaque Quraishi, Shailesh Kumar Jadhav and Sheetal Gupta
Cassia tora L. is a leguminous annual herb of tropical part of India. It is an important medicinal plant with commercially valuable seeds. Micropropagation of this medicinal legume via bud culture method has been first time reported here. Nodal explants from field grown plants were used to initiate in vitro culture. Heavy black leaching was observed in initiation medium from cut ends of the explants and all the explants were eventually died. Leaching was effectively checked by incorporation of an absorbent and an antioxidant -25 μM polyvinyl pyrrolidone -40 and 476 μM citric acid in the initiation medium, respectively. Best 90% shoot bud initiation with 1.5±0.1 shoots per explant was recorded on MS medium supplemented with 2.2 μM 6-benzyl adenine. Elongated shoots from original explants were divided into nodes and best shoot production was observed on MS basal medium, which also induced roots from the propagule. These shoots on the basal medium were longest (3.3 cm±0.38) also with highest number of nodes. There was no leaching observed at this stage therefore the mixture of polyvinyl pyrrolidone -40 and citric acid was not incorporated into the medium. For better quality roots and 100% rooting in shoots, elongated shoots were transferred into ½ strength MS medium supplemented with 2.5 or 4.9 μM indole-3-butyric acid. Regenerated plantlets were successfully hardened off in greenhouse with 70% survival.
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