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Biotechnology

Year: 2011 | Volume: 10 | Issue: 6 | Page No.: 534-539
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Authors

Vandana Kashyap

Bijaya Ketan Sarangi

Manoj Kumar Yadav

Dinesh Yadav

Keywords

Research Article

Assessment of In vitro Multiple Shoot Bud Induction from Leaf Explants among Eleven Indian Cultivars of Pigeon Pea (Cajanus cajan L. Mill sp.)

Vandana Kashyap, Bijaya Ketan Sarangi, Manoj Kumar Yadav and Dinesh Yadav
In vitro multiple shoot bud induction from leaf explants of eleven Indian cultivars of pigeon pea (Cajanus cajan L. Mill sp.) under the influence of variable concentration of three different cytokinins namely BAP, KIN and TDZ on MS media has been studied. Callus of variable morphology and nature was observed for most of the cultivars except four cultivars IPA-2013, Pusa-9, T-7 and IPA-98-3 showing multiple shoot bud induction. Among cytokinins used BAP was found to be most effective. Further elongation of multiple shoot buds was achieved in the same media while rooting of regenerated multiple shoots were attempted in MS media supplemented with variable concentration of three different auxins namely NAA, IAA and IBA. Efficient rooting was achieved in the presence of NAA. Regenerated plants were successfully established in soil with acclimatization frequency varying from 25-50% with cultivar IPA-2013 showing the best response.
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