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Biotechnology

Year: 2011 | Volume: 10 | Issue: 6 | Page No.: 514-520
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Authors

C. Nathanailides

T. Chanzaropoulos

A. Barbouti

C. Perdikaris

T. Zhang

Keywords

Research Article

DNA Fragmentation, Linear Velocity and Fertilising Ability of Reactivated Cryopreserved Goldfish Sperm using Different Cryoprotectants

C. Nathanailides, T. Chanzaropoulos, A. Barbouti, C. Perdikaris and T. Zhang
The motility, fertilising ability and DNA integrity of freeze-thawed goldfish sperm was evaluated using computer-aided sperm motility analysis and the comet assay. The results indicate that sublethal effects during the freeze-thawing procedure compromised sperm fertilising ability, DNA integrity, velocity linearity and the duration of swimming. Compared to 10% Ethylene glycol (EG), 5% and 10% Egg yolk (EY) and DMSO treated sperm exhibited higher percentage of motile sperm and higher path velocity (VAP); straight-line velocity (VSL) linearity (LIN), DNA integrity and fertilising ability. There was a low but significant (r = 0.67, p<0.05) correlation between DNA integrity and the maximum Duration of Forward Progressive Swimming (DFPS) ability of cryopreserved goldfish sperm. DNA integrity and to a lesser degree the simple assessment of the DFPM of thawed sperm may be reliable indicators of sperm integrity for the development and the rapid assessment of fish sperm cryopreservation protocols.
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