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Biotechnology

Year: 2010 | Volume: 9 | Issue: 1 | Page No.: 9-16
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Authors

Naulchan Khongsay

Lakkana Laopaiboon

Pattana Laopaiboon

Keywords

Research Article

Growth and Batch Ethanol Fermentation of Saccharomyces cerevisiae on Sweet Sorghum Stem Juice under Normal and Very High Gravity Conditions

Naulchan Khongsay, Lakkana Laopaiboon and Pattana Laopaiboon
Saccharomyces cerevisiae NP 01 was grown on sweet sorghum stem juice under normal gravity (NG, total sugar of 240 g L-1) and very high gravity (VHG, total sugar of 280 g L-1) conditions. The effects of initial cell concentrations (1x106, 1x107 and 1x108 cells mL-1) and nitrogen supplementation [0.5% (NH4)2SO4] on growth and ethanol production were investigated. The experiments were carried out at 30°C in a 2-L fermenter under an agitation rate of 100 rpm. Under both NG and VHG fermentations, the initial cell concentration of 1x108 cells mL-1 and sweet sorghum stem juice without nitrogen supplementation gave the maximum ethanol production efficiencies. The concentration, productivity and yield of ethanol were 105.12±1.34 g L-1, 2.92±0.04 g L-1 h-1 and 98.33±1.25% of theoretical yield, respectively at the fermentation time of 36 h under the NG condition. Under the VHG fermentation, the maximum ethanol concentration was not varied from that of the NG conditions, whereas the productivity and yield of ethanol were significantly lower than those of the NG conditions.
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