Abstract:
Micropropagation has been extensively used for the rapid multiplication of many plants species. However, its wider use is restricted often by the high percentage of plant loss or damaged when transferred to ex vitro condition. To acclimatize the micropropagated plants, different workers have employed different approach toward successful establishment of in vitro raised plants under ex vitro condition. In the present study, a successful attempt has been made to acclimatize the tissue culture raised plants which is cost effective compare to other existing hardening technique. Tissue culture raised orchid seedlings were acclimatized and hardened in vitro by using 1/10th liquid MS basal medium subsequently replaced by tap water with chips of charcoal, bricks and decayed wood as an alternate substratum. The newly formed roots under this condition firmly attached to the charcoal chips with the passage of time, similar to velamenous root attach to the trunk of a tree. Charcoal and moss substratum was more suitable than bricks for epiphytic species and decayed woods for terrestrial. The researchers report the novel and efficient one step hardening technique for tissue culture raised orchid seedlings which will reduce the production cost.
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