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Biotechnology
  Year: 2010 | Volume: 9 | Issue: 3 | Page No.: 378-382
DOI: 10.3923/biotech.2010.378.382
Isolation and Characterization of Biosurfactant Producing Bacteria from Caspian Sea
A. Safary, M. Roayayi Ardakani, A. Abolhasani Suraki, M. Akbarzade Khiavi and H. Motamedi

Abstract:
Marine biosurfactants produced by some marine microorganisms have been paid more attention, particularly for the bioremediation of the sea polluted by crude oil. The goal of this study was isolation and characterization of biosurfactant producing bacteria from Caspian Sea water. Ten morphologically distinct microbial colonies were isolated and screened for biosurfactant production. From the results, tow strains isolated from Caspian Sea in Iran, which were able to grow on crud oil as sole carbon sources and to produce biosurfactants. Primary screening of biosurfactant-producing colonies was performed using the qualitative drop-collapse test, oil displacement test and hemolytic test. Among tow strains CpA1 completely emulsified crud oil in MSM medium within 48 h of cultivation. This strain exhibited the highest activity for oil displacement test toward crud oil (3.14 cm2) and emulsification activity. In second screening, the surface tension of culture supernatants for CpA1 isolate grown under identical conditions was measured by Captive Drop Cell instrument. This strain reduced the growth medium surface tension below 40 mN m-1 and selected as a best biosurfactant producer in this study.
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How to cite this article:

A. Safary, M. Roayayi Ardakani, A. Abolhasani Suraki, M. Akbarzade Khiavi and H. Motamedi, 2010. Isolation and Characterization of Biosurfactant Producing Bacteria from Caspian Sea. Biotechnology, 9: 378-382.

DOI: 10.3923/biotech.2010.378.382

URL: https://scialert.net/abstract/?doi=biotech.2010.378.382

 
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