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Biotechnology
  Year: 2010 | Volume: 9 | Issue: 2 | Page No.: 224-228
DOI: 10.3923/biotech.2010.224.228
 
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Saccharification of Raw Native Starches by Extracellular Isoamylase of Rhizopus oryzae

B. Ghosh and R.R. Ray

Abstract:
Extra cellular isoamylase from Rhizopus oryzae PR7 MTCC 9642 was found to saccharify soluble potato starch and various native raw starches collected from domestic effluents, of which arrow root, tamarind kernel, tapioca and oat were noteworthy. In the present study, the effects of incubation time, pH, temperature, substrate concentration, gelatinization and chemical pretreatment of starches on saccharification were quantified in detail. Highest extent of saccharification was found at 55°C. Except tamarind kernel powder, the best pH for bioconversion of all other substrates was found to be at 5.0. The maximum amount of bioconversion was accomplished within 60 min of incubation with a substrate concentration ranging from 0.5 to 1% (w/v). About 85-200% increase in sugar production was found after thermal pretreatment of the starches. Amongst the chemicals, Mn2+ brought about a uniform increase in sugar production for all the substrates used, whereas thiol compounds could also increase the extent of saccharification.
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How to cite this article:

B. Ghosh and R.R. Ray, 2010. Saccharification of Raw Native Starches by Extracellular Isoamylase of Rhizopus oryzae. Biotechnology, 9: 224-228.

DOI: 10.3923/biotech.2010.224.228

URL: https://scialert.net/abstract/?doi=biotech.2010.224.228

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