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Biotechnology

Year: 2010 | Volume: 9 | Issue: 2 | Page No.: 141-148
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Authors

R. Kondamudi

V. Vijayalakshmi

K. Sri Rama Murthy

Keywords

Research Article

Induction of Morphogenetic Callus and Multiple Shoot Regeneration in Ceropegia pusilla Wight and Arn.

R. Kondamudi, V. Vijayalakshmi and K. Sri Rama Murthy
This study was undertaken to evaluate the most suitable concentration of plant growth regulators and perfect explant (node, internode and thin cell layer explants-TCLs) for callus induction and subsequent organogenesis in an endangered medicinal Ceropegia pusilla. The best callus induction was found on the MS medium supplemented with 6-benzylaminopurine (BAP) 13.32 μM L-1 + 2, 4-dichlorophenoxy acetic acid (2,4-D) 0.45 μM L-1 from TCLs. After the initiation of the callus, it was immediately transferred to MS medium supplemented with BAP along with other auxins like 2, 4-D, Indol-3-Acetic Acid (IAA), Indole-3-Butyric Acid (IBA), Naphthalene Acetic Acid (NAA). The regenerative calli were raised on the MS medium supplemented with 1.13 μM L-1 of 2,4-D. Whereas, the organogenic calli was raised on the medium containing 22.7, 40.86, 45.4 μM L-1 of Thidiazuron (TDZ) induced 37.54±0.29, 37.12±0.18 and 34.32±0.17 shoots, respectively. On the media containing BAP 13.32 + IBA 0.49 to 1.23 μM L-1 micro shoots rooted best and 75% of the shoots were survived. The plantlets were established, acclimatized and thrived in green house conditions with 80%. The regeneration protocol developed in this study provides a basis for germplasm conservation and for further investigation of bio active constituents of this medicinal plant.
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