Research Article

**Application of Protease Isolated from Bacillus sp. 158 in Enzymatic Cleansing of Contact Lenses**

Rasika Pawar, Vasudeo Zambare, Siddhivinayak Barve and Govind Paratkar

A neutral protease, isolated from Bacillus sp. 158 was used for removing protein deposits from contact lenses. Partial purification of the protease was carried out using ammonium sulphate and factors affecting the enzyme activity, such as assay temperature and assay pH were characterized. The optimum pH and temperature for protease were found to be pH 7.0 and 30°C, respectively. The partially purified protease was stable at temperature range of 30-40°C and pH 6-7. However, protease was maximum stable at 30°C and pH 7.0. The enzyme could be effectively used to remove protein deposit from contact lenses indicating its potential to increase in transmittance of lenses.

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How to cite this article

Rasika Pawar, Vasudeo Zambare, Siddhivinayak Barve and Govind Paratkar, 2009. Application of Protease Isolated from Bacillus sp. 158 in Enzymatic Cleansing of Contact Lenses. Biotechnology, 8: 276-280.

DOI: 10.3923/biotech.2009.276.280

URL: https://scialert.net/abstract/?doi=biotech.2009.276.280

Comments

Archana Bhole Reply

12 April, 2009

The paper is nicely written by authors and is well managed research pointof view.

Sandeep Chaudhary Reply

15 April, 2009

Reserch Paper theme is very good with good liturature survey, summarization and writting skills.

Mangesh Ahire Reply

17 December, 2018

Nice article

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Authors

Rasika Pawar

Vasudeo Zambare

Siddhivinayak Barve

Govind Paratkar

Keywords