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Biotechnology
  Year: 2009 | Volume: 8 | Issue: 1 | Page No.: 176-179
DOI: 10.3923/biotech.2009.176.179
 
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Study of Expression of Low-Temperature-Responsive Genes for Selected Barley Accessions
M. Keykhahsaber, A.A.V. Sedehi, A. Zakeri, H. Khaje and Z. Keykhah

Abstract:
In order to determine the level of low temperature response genes expression in ten selected barley accessions were obtained from Sistan Agricultural Research at spring of 2008. RNA from each of barley germination was extracted in two cold acclimation and one control condition. The result of real time PCR, using cDNA from three low temperature response gene (bit2, bit14 and bit101), showed that there were significant difference in gene expression between three treatments and in each gene the highest percentage of gene expression belonged to accession 5 while accession 8, 9 and 10 composed one separate. In bit14 gene, the increase in the amount of mRNA was carried out when the maximum level of freezing (4 °C day/2 °C night) apply. All of these genes are shown to be transcriptional regulated and root meristem had maximum level of RNA under cold treatment.
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How to cite this article:

M. Keykhahsaber, A.A.V. Sedehi, A. Zakeri, H. Khaje and Z. Keykhah, 2009. Study of Expression of Low-Temperature-Responsive Genes for Selected Barley Accessions. Biotechnology, 8: 176-179.

DOI: 10.3923/biotech.2009.176.179

URL: https://scialert.net/abstract/?doi=biotech.2009.176.179

 
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