Abstract:
This research aims to select and evaluate the performance
of three high-ethanol-producing strains of Saccharomyces cerevisiae(TISTR 5048, TISTR 5339 and NP 01) in very high gravity (VHG) ethanol
fermentation. The maximum specific growth rates (μmax)
of TISTR 5048 and NP 01 grown in yeast extract malt extract broth containing
150 g glucose L-1 were 0.49 and 0.46 h, respectively while
μmax of TISTR 5339 could not be determined due to cell
flocculation. The ethanol production by TISTR 5048 and NP 01 was further
carried out in batch mode at 30 °C under normal gravity fermentation
(240 g glucose L-1) and VHG fermentation (280 and 320 g glucose
L-1) and the initial cell concentration was 1x108
cells mL-1. The results showed that TISTR 5048 cultured in
240 and 280 g glucose L-1 gave the maximum ethanol concentration
(P) with the value of 99.58 g L-1, while NP 01 cultured in
280 and 320 g glucose L-1 gave the maximum P with the value
of 104.68 g L-1. Ethanol productivities (Qp) of
NP 01 were slightly higher than those of TISTR 5048 at all conditions
tested.
Lakkana Laopaiboon, Sunan Nuanpeng, Penjit Srinophakun, Preekamol Klanrit and Pattana Laopaiboon, 2008. Selection of Saccharomyces cerevisiae and Investigation of its Performance for Very High Gravity Ethanol Fermentation. Biotechnology, 7: 493-498.
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