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Biotechnology
  Year: 2008 | Volume: 7 | Issue: 3 | Page No.: 439-447
DOI: 10.3923/biotech.2008.439.447
The Use of Trichoderma harzianum and T. viride as Potential Biocontrol Agents Against Peanut Microflora and Their Effectiveness in Reducing Aflatoxin Contamination of Infected Kernels
Emma W. Gachomo and Simeon O. Kotchoni

Abstract:
The antagonistic mechanism used by four Trichoderma isolates against peanut moulds; Aspergillus flavus, A. parasiticus, A. niger, A. ochraceous and Fusarium species was investigated under laboratory conditions. Two isolates of T. harzianum namely Th1 and Th2 and two isolates of T. viride, Tv1 and Tv2, were found to efficiently suppress the growth of peanut moulds and to significantly reduce aflatoxins, AFB1 and AFB2, contents in infected peanut kernels. The degree to which Trichoderma species suppressed the growth of peanut moulds correlated to their extracellular enzymatic activities. The Trichoderma isolates displayed various extracellular enzymatic activities including amylolytic, chitinolytic, pectinolytic, proteolytic, lipolytic and cellulolytic to fight off the other fungal competitors in the media. The more diverse the enzymatic activities, the more effective are the antagonists in suppressing the growth of peanut moulds and aflatoxin production in kernels. Present results suggest that Trichoderma-enzymolomic can be exploited as potential antimicrobial candidates against peanut moulds and the subsequent aflatoxin production in peanut kernels.
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How to cite this article:

Emma W. Gachomo and Simeon O. Kotchoni, 2008. The Use of Trichoderma harzianum and T. viride as Potential Biocontrol Agents Against Peanut Microflora and Their Effectiveness in Reducing Aflatoxin Contamination of Infected Kernels. Biotechnology, 7: 439-447.

DOI: 10.3923/biotech.2008.439.447

URL: https://scialert.net/abstract/?doi=biotech.2008.439.447

 
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