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Biotechnology
  Year: 2008 | Volume: 7 | Issue: 2 | Page No.: 328-332
DOI: 10.3923/biotech.2008.328.332
 
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Direct Shoot Formation and Microtuberization from Aseptic Seedlings of Cyclamen mirabile Hildebr.

Omer Yamaner and Bengi Erdag

Abstract:
In this study, an efficient propagation method was developed for Cyclamen mirabile Hildebr., an endangered endemic species having ornamental importance in Turkey. Sterilized seeds of C. mirabile were cultured on full and half-strength (½) Murashige and Skoog (MS) basal media for germination and seedling growth. Tissues excised from 8 week-old seedlings were used as explant source for shoot regeneration and microtuberization. Shoots were obtained from only tuber slices on Bourgin-Nitsch (BN) medium supplemented with auxin and cytokinin. The largest of number of shoots (12.2 shoots/explant) was obtained on ½ MS medium containing 1 mg L-1 NAA and 0.5 mg L-1 kinetin. Microtubers were formed in the medium promoting shoot proliferation. The highest microtuber formation was obtained on the medium containing 2 mg L-1 BA plus 0.1 mg L-1 NAA.
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How to cite this article:

Omer Yamaner and Bengi Erdag, 2008. Direct Shoot Formation and Microtuberization from Aseptic Seedlings of Cyclamen mirabile Hildebr.. Biotechnology, 7: 328-332.

DOI: 10.3923/biotech.2008.328.332

URL: https://scialert.net/abstract/?doi=biotech.2008.328.332

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