Subscribe Now Subscribe Today
Science Alert
 
FOLLOW US:     Facebook     Twitter
Blue
   
Curve Top
Biotechnology
  Year: 2007 | Volume: 6 | Issue: 1 | Page No.: 93-96
DOI: 10.3923/biotech.2007.93.96
 
Facebook Twitter Digg Reddit Linkedin StumbleUpon E-mail
Conserved Region Analysis of Oncogenic Human Papillomavirus Genome
Usman Sumo Friend Tambunan , Herbert Wybert Butar-Butar , Radya Umbas and Zulfa Hidayah

Abstract:
This study was carried out to determine the conserved regions of late genes from sequenced HPV types. HPV genome sequences were collected from the Los Alamos National Laboratory papillomavirus database. There are 74 types of HPV in the database which have completely documented genome sequences as well as their translation product. Specific types of HPV which may cause cervical cancer are grouped into high risk or low risk, according to their risk potential. This classification may differ from one research methodology to another. In order to access a representative classification, three sets of classification were studied for this research. HPV type 16 and 18 are consistently grouped as high risk, while other types of HPV varied randomly. Sequence alignment was taken and the result shows 62 conserved regions as a primer template for L1 and L2 genes. These conserved regions were then subjected to BLASTn operation in order to search the conserved region with least similarity to low risk HPV and human genome. Finally, 7 selected conserved regions were examined for secondary structures using NetPrimer program. From this operation, only region 52 (5’-ACAGGCTATGGTGCTATGGA-3’) met the criteria to be used as an oligonucleotide primer.
PDF Fulltext XML References Citation Report Citation
 RELATED ARTICLES:
  •    Parallel Particle Swarm Optimization for Global Multiple Sequence Alignment
  •    Association of Pap Smear Abnormalities with Autoimmune Disorders
How to cite this article:

Usman Sumo Friend Tambunan , Herbert Wybert Butar-Butar , Radya Umbas and Zulfa Hidayah , 2007. Conserved Region Analysis of Oncogenic Human Papillomavirus Genome. Biotechnology, 6: 93-96.

DOI: 10.3923/biotech.2007.93.96

URL: https://scialert.net/abstract/?doi=biotech.2007.93.96

 
COMMENT ON THIS PAPER
 
 
 

 

 
 
 
 
 
 
 
 
 

 
 
 
 
 
 
 

       

       

Curve Bottom