Subscribe Now Subscribe Today
Science Alert
 
Blue
   
Curve Top
Biotechnology
  Year: 2007 | Volume: 6 | Issue: 4 | Page No.: 520-526
DOI: 10.3923/biotech.2007.520.526
 
Facebook Twitter Digg Reddit Linkedin StumbleUpon E-mail

Extraction of Dengue 2 Plasmid DNA Vaccine (pD2) from Cell Lysates by Aqueous Two-Phase Systems

Keila Aparecida Moreira, Adilson Castro Chaves, Ernesto Torres Marques, Duarte Miguel Ferreira Prazeres, Walter Mendes de Azevedo, Ana Lucia Figueiredo Porto and Jose Luiz de Lima Filho

Abstract:
This research describes the partitioning in PEG/phosphate systems of the plasmid pD2, a dengue 2 plasmid DNA vaccine, present in a clarified E. coli alkaline lysate. Factors that affect the partition as PEG molecular weight, plasmid concentration and the lysate volume loaded in the system were investigated. Results showed that partition behavior of plasmid DNA depends on the system molecular weight, a considerable amount of protein of the cell lysate was accumulated in the interphase of the systems. The best recovery plasmid yield (37%) was obtained with PEG 400 (20/20% w/w) systems with a 60% (w/w) lysate load.
PDF Fulltext XML References Citation Report Citation
 RELATED ARTICLES:
  •    How to Cope with Dengue in Developing Countries Like Pakistan?
How to cite this article:

Keila Aparecida Moreira, Adilson Castro Chaves, Ernesto Torres Marques, Duarte Miguel Ferreira Prazeres, Walter Mendes de Azevedo, Ana Lucia Figueiredo Porto and Jose Luiz de Lima Filho, 2007. Extraction of Dengue 2 Plasmid DNA Vaccine (pD2) from Cell Lysates by Aqueous Two-Phase Systems. Biotechnology, 6: 520-526.

DOI: 10.3923/biotech.2007.520.526

URL: https://scialert.net/abstract/?doi=biotech.2007.520.526

COMMENT ON THIS PAPER
 
 
 

 

 
 
 
 
 
 
 
 
 

 
 
 
 
 
 
 

Curve Bottom