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Biotechnology
  Year: 2007 | Volume: 6 | Issue: 3 | Page No.: 440-443
DOI: 10.3923/biotech.2007.440.443
 
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Isolation and Partial Purification of Extracellular Enzyme (1, 3)-β-D Glucanase from Trichoderma reesei (3929)
R. Saravanan, V. Pavani Devi, A. Shanmugam and D. Sathish Kumar

Abstract:
The fungus Trichoderma reesei produces extracellular lytic enzymes such as β-1, 4 glucanases, β-1, 6 glucanases and β-1, 3 D glucanases. In the present study the PDA medium was used for the production of β-1, 3 glucanases from the fungal strain. Extra cellular protein (β-1, 3 glucanase) was partially purified by ammonium sulphate precipitation and dialysis. The amount of reducing sugar (126 mg/100 mL) liberated by the action of enzyme was determined by using DNS method. The molecular weight of the enzyme (52-62 kDa) was determined through SDS-PAGE.
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How to cite this article:

R. Saravanan, V. Pavani Devi, A. Shanmugam and D. Sathish Kumar, 2007. Isolation and Partial Purification of Extracellular Enzyme (1, 3)-β-D Glucanase from Trichoderma reesei (3929). Biotechnology, 6: 440-443.

DOI: 10.3923/biotech.2007.440.443

URL: https://scialert.net/abstract/?doi=biotech.2007.440.443

 
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