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Biotechnology

Year: 2007 | Volume: 6 | Issue: 3 | Page No.: 426-430
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Authors

Mohamed M.M. Ahmed

Salah M. Abdel-Rahman

Amr A. El-Hanafy

Keywords

Research Article

Application of Species-Specific Polymerase Chain Reaction and Cytocrome b Gene for Different Meat Species Authentication

Mohamed M.M. Ahmed, Salah M. Abdel-Rahman and Amr A. El-Hanafy
DNA was extracted from buffalo’s, cattle’s, pig’s and sheep’s muscles and Polymerase Chain Reaction (PCR) technique was used for detection, identification and authentication in raw meat samples by using Species-Specific Repeat (SSR). The PCR amplification size of the gene encoding SSR region in buffalo’s, cattle’s, pig’s and sheep’s meat were 603, 603, ≤100 and 374 bp, respectively. For discrimination between buffalo’s and cattle’s meat, amplified cytocrome b gene (359 bp) was digested by TaqI restriction enzyme. Two fragments 191 and 168 bp were generated in buffalo, whereas digested not with cattle (359 bp). The results showed that SSR and cytocrome b gene PCR-RFLP analysis provide a rapid and effective methods to detect the meat species and it could be easily identified and authenticated. In addition, the SSR PCR and cytocrome b gene PCR-RFLP methods are highly sensitive and will improve the detection limits for DNA sequences derived from these species. This finding could be easily used for authentication and detection of imported mad cow meat (lunacy) from origin countries.
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