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  Year: 2007 | Volume: 6 | Issue: 2 | Page No.: 299-301
DOI: 10.3923/biotech.2007.299.301
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In vitro Propagation of Oroxylum indicum-An Endangered Medicinal Tree

Sharad Tiwari, Kanchan Singh and Pankaj Shah

Seedling stem sections of Oroxylum indicum Vent. were cultured on Murashige and Skoog (MS) medium with or without growth regulators 6-benzylaminopurine and 1-naphthaleneacetic acid for proliferation and growth of shoots. Callus proliferation started after 2-weeks of initial culture on all the media supplemented with growth regulators, being maximum (>90%) in the treatment having 3.0 mg L-1 6-benzyaminopurine and 0.5 mg L-1 1-naphthaleneacetic acid. Subculture of seedling stem sections after 4-weeks on the shoot initiation medium led to multiple shoot induction during the next 4-weeks. Utmost, 70.8% explants initiated shoots at an average of 61.1 shoots per explant on the medium found best for callus proliferation. The most congenial rooting medium was half-strength MS medium plus 0.5 mg L-1 indole-3-butyric acid. The rooted plants were successfully transferred to soil, exhibiting a normal development.
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How to cite this article:

Sharad Tiwari, Kanchan Singh and Pankaj Shah, 2007. In vitro Propagation of Oroxylum indicum-An Endangered Medicinal Tree. Biotechnology, 6: 299-301.

DOI: 10.3923/biotech.2007.299.301






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