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Biotechnology
  Year: 2007 | Volume: 6 | Issue: 1 | Page No.: 101-104
DOI: 10.3923/biotech.2007.101.104
Regeneration of Ginger Plant from Callus Culture Through Organogenesis and Effect of CO2 Enrichment on the Differentiation of Regenerated Plant
Muhammad Jamil , Jin Key Kim , Zahid Akram , Saif Ullah Ajmal and Eui Shik Rha

Abstract:
A efficient and systematic protocol for complete plant regeneration via shoot apical meristem culture has been developed for Zingiber officinale L. Callus was initiated from shoot-tip of young plant on MS-media supplemented with a combination of Naphthalene acetic acid (0.1 mg L-1) and Kinetin (1.0-2.0 mg L-1) and Indole-3-acetic acid (0.1 mg L-1) and 6-Benzylaminopurine (1.0-2.0 mg L-1). Maximum shoot differentiation from callus occurred on MS medium supplemented with Indole-3-acetic acid (0.1 mg L-1) and 6-Benzylaminopurine (1.0 mg L-1). Complete plantlets were transferred into specially made plastic pot containing soilrite followed by their transfer to the field soil. Survival rate of the plantlets under ex vitro condition was 90%. Regenerated plants on MS medium supplemented with Naphthalene acetic acid (0.1 mg L-1) and 6-Benzylaminopurine (0.1 mg L-1) was exposed to elevated CO2 concentration. A 400-4000 ppm increase in atmospheric CO2 concentration led to an increase in adventitious bud and shoot primodium while the growth of adventitious bud and shoot primodium were reduced at 8000 ppm.
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How to cite this article:

Muhammad Jamil , Jin Key Kim , Zahid Akram , Saif Ullah Ajmal and Eui Shik Rha , 2007. Regeneration of Ginger Plant from Callus Culture Through Organogenesis and Effect of CO2 Enrichment on the Differentiation of Regenerated Plant. Biotechnology, 6: 101-104.

DOI: 10.3923/biotech.2007.101.104

URL: https://scialert.net/abstract/?doi=biotech.2007.101.104

 
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