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Biotechnology
  Year: 2006 | Volume: 5 | Issue: 1 | Page No.: 58-61
DOI: 10.3923/biotech.2006.58.61
 
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A PCR-RFLP Method for the Analysis of Egyptian Goat MHC Class II DRB Gene
Sahar Ahmed and Othman E. Othman

Abstract:
This study aimed to analysis the genetic polymorphisms of MHC class II DRB gene in the Egyptian goat using PCR-RFLP method. The amplified fragment with size of 285-bp was digested by two restriction enzymes TaqI and PstI. Restriction digestion of PCR product by TaqI enzyme represented two digested fragments at 122- and 163-bp (T restriction pattern) or undigested fragment at 285-bp (t restriction pattern). The frequencies of TT, Tt and tt patterns of MHC class II DRB in the Egyptian goats were 29.5, 61.4 and 9.1%, respectively. After the PstI digestion of the 285-bp PCR products amplified by Egyptian goat DNA, the results showed that the frequency of pp pattern (270- and 15-bp restricted fragments) was 29.5% and the frequency of heterozygous Pp pattern (270-, 226-, 44- and 15-bp restricted fragments) was 70.5%, while the PP pattern (226-, 44- and 15-bp restricted fragments) was not displayed in tested goat animals. The results declared that the p pattern (64.8%) is dominant over the P pattern (35.2%) in Egyptian goat.
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How to cite this article:

Sahar Ahmed and Othman E. Othman , 2006. A PCR-RFLP Method for the Analysis of Egyptian Goat MHC Class II DRB Gene. Biotechnology, 5: 58-61.

DOI: 10.3923/biotech.2006.58.61

URL: https://scialert.net/abstract/?doi=biotech.2006.58.61

 
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