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Biotechnology
  Year: 2006 | Volume: 5 | Issue: 1 | Page No.: 49-52
DOI: 10.3923/biotech.2006.49.52
Application of In vitro Tissue Culture Techniques for Propagation of Amygdalus communis L. Cultivars, Garrigues and Yaltsinki
Haki Yapar, Canan Can , Mehmet Ozaslan , Turkan Aytekin and Halit Atli

Abstract:
Almond (Amygdalus communis L.) is planted and naturally spreads in the Southeastern Anatolian region where some hybrid varieties are cultivated and the adaptation studies have still been continued. Although being easily propagated through grafting, plantation in large areas may have some problems, due to the environmental stress. Embryos, nodal segmets of mature shoots and shoot tips of two A. communis L. cultivars, Garrigues and Yaltsinki, were cultured on MS media supplemented with several concentrations and combinations of cytokinins and auxins, for in vitro micropropagation. The best root formation in embryo cultures for both cultivars was obtained in the media without growth regulators. The highest stem development was obtained with 4.0 mg L-1 of IAA in Garrigues. Callus developed from cultured shoot tips. MS media supplemented with 4.0 mg L-1 BAP induced fast growing-fragile callus growth. The best nodal explant development occurred with MS contained 2.0 mg L-1 BAP and 0.5 mg L-1 IAA. Results obtained demonstrated that the nodal explants could be used for direct embryogenesis of Garrigues and Yaltsinki varieties.
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How to cite this article:

Haki Yapar, Canan Can , Mehmet Ozaslan , Turkan Aytekin and Halit Atli , 2006. Application of In vitro Tissue Culture Techniques for Propagation of Amygdalus communis L. Cultivars, Garrigues and Yaltsinki. Biotechnology, 5: 49-52.

DOI: 10.3923/biotech.2006.49.52

URL: https://scialert.net/abstract/?doi=biotech.2006.49.52

 
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