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Biotechnology

Year: 2004 | Volume: 3 | Issue: 1 | Page No.: 75-81
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Authors

G.H. Abro

T.S. Syed

G.M. Tunio

M.A. Khuhro

Keywords

Research Article

Performance of Transgenic Bt Cotton Against Insect Pest Infestation

G.H. Abro, T.S. Syed, G.M. Tunio and M.A. Khuhro
Studies were carried out to investigate insect infestation on transgenic Bt and non-Bt cotton varieties and genotypes. The seeds of seven cotton varieties, viz., KMG-1, KMG-2, KMG-3, MS-1, MS-2 NIAB-78 and CRIS-134 were sown on May 15, 2002 in a completely randomized block design. The study comprised laboratory bioassay and field screening of different varieties and genotypes. Laboratory study indicated that transgenic Bt cotton was highly toxic to Earias vittella causing 100% larval mortality. While armyworm, Spodoptera exigua was tolerant, its mortality ranged between 13.3 to 53.3% on different varieties and genotypes. The results of field study revealed that the highest mean population (1.66 insect per leaf) of jassid was recorded on KMG-3 followed by 1.95 and 1.52 insects leaf on KMG-2 and KMG-1, respectively. Whitefly population ranged between 1.47 and 1.52 insects leaf on different varieties and genotypes. Whereas, thrips population remained fluctuating in different varieties and genotypes during the crop growth period. But genotypes like KMG-1, KMG-2 and KMG-3 which are crosses of Bt cotton and non-Bt cotton varieties were found harboring more thrips population. The larval population of spotted bollworm, Earias spp. indicated no infestation and live larvae were found on MS-1 and MS-2 varieties of Bt cotton. Maximum infestation of spotted bollworm was recorded on NIAB-78 (0.97 larva per plant) followed by other varieties and genotypes.
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