Rapid and Simultaneous Identification of Two Salmonella enterica Serotypes, Enteritidis and Typhimurium from Chicken and Meat Products by Multiplex PCR


	Biotechnology
	Year: 2004 \| Volume: 3 \| Issue: 1 \| Page No.: 44-48 DOI: 10.3923/biotech.2004.44.48

Rapid and Simultaneous Identification of Two Salmonella enterica Serotypes, Enteritidis and Typhimurium from Chicken and Meat Products by Multiplex PCR

Hanan Issa Malkawi and Raad Gharaibeh

Abstract: In order to overcome the large volume of work required to detect and identify food samples that may contain Salmonella enterica serotypes Enteritidis and Typhimurium, multiplex polymerase chain reaction (m-PCR) was used as a diagnostic tool to identify Salmonella enterica, Salmonella enterica ser. Enteritidis and Typhimurium in naturally contaminated meat and poultry products. Three sets of known S. enterica and serotypes S. enteritidis and S. typhimurium specific primers were applied to simultaneous identification of such pathogens in the most frequently used fresh and frozen meat (beef and lamb) and poultry (chicken) products (whole, cut, ground and processed) collected from eight locations within Irbid city (Jordan). Out of 93 samples, Only 21 (22.5%) samples contained serotype S. typhimurium alone. Nineteen samples (20%) showed the 312 bp specific band for serotype S. enteritidis specific band. A total of 28 (30%) samples showed only for S. enterica genus and 25 (26.8%) samples showed both serotypes S. enteritidis and S. typhimurium. In general, 46 (49.5%) samples contained serotype S. typhimurium, while 44 (47.3%) samples contained serotype S. enteritidis. Multiplex PCR amplification was shown to be an effective and rapid method for the simultaneous identification of such pathogens in food samples.

PDF References Citation Report Citation

RELATED ARTICLES:

•	Advances in the Research of AMPK and its Subunit Genes

•	Multiplex PCR (Polymerase Chain Reaction) Assay for Detection of E. coli O157:H7, Salmonella sp., Vibrio cholerae and Vibrio parahaemolyticus in Spiked Shrimps (Penaeus monodon)

•	Development of Multiplex PCR (Polymerase Chain Reaction) Method for Detection of Salmonella spp. and Vibrio parahaemolyticus from Shrimp Samples of Bangladesh

•	Qualitative and Quantitative Validation of the Micro Biological Survey Method for Listeria spp., Salmonella spp., Enterobacteriaceae and Staphylococcus aureus in Food Samples

How to cite this article:

Hanan Issa Malkawi and Raad Gharaibeh, 2004. Rapid and Simultaneous Identification of Two Salmonella enterica Serotypes, Enteritidis and Typhimurium from Chicken and Meat Products by Multiplex PCR. Biotechnology, 3: 44-48.

DOI: 10.3923/biotech.2004.44.48

URL: https://scialert.net/abstract/?doi=biotech.2004.44.48

COMMENT ON THIS PAPER

	Full Name:
	E-mail:
	Comments:

Navigation


		Online First
		Current Issue
		Previous Issues
		Editorial Board
		Submit a Manuscript
		Guide to Authors
		Subscribe to E-alerts

Indexed In


		AGRICOLA
		AGRIS
		ASCI-Database
		Asian Digital Library
		Cambridge Scientific Abstract

Follow @scialert

Science Alert