Subscribe Now Subscribe Today
Science Alert
 
FOLLOW US:     Facebook     Twitter
Blue
   
Curve Top
Biotechnology
  Year: 2004 | Volume: 3 | Issue: 2 | Page No.: 173-180
DOI: 10.3923/biotech.2004.173.180
Comparative Study of Probiotic Cultures to Control the Growth of Escherichia coli O157: H7 and Salmonella typhimurium
Moustafa Y.M. El-Naggar

Abstract:
Pure lactobacilli (Lactobacillus acidophilus, L. plantarum and L. brevis) strains isolated from commercial probiotic consortium (TrufermTM) and Bacillus subtilis ATCC 6633 were investigated for their in vitro antagonistic ability against two enteric pathogens (Escherichia coli O157:H7 and Salmonella typhimurium). Generally, both pathogens were inhibited by the probiotic strains but the inhibition of S. typhimurium was greater than that of E. coli. Furthermore, B. subtilis had a better antagonistic activity compared to that recorded for lactobacilli. Among lactobacilli, L. acidophilus exhibited the highest antagonistic activity against both indicator strains. All probiotic strains succeeded to exhibit a positive coaggregation with both faecal pathogens. The maximum coaggregation was reached after 4 h of incubation of indicator strains with lactobacilli and 6 h with B. subtilis. L. acidophilus recorded the highest coaggregation result. Regarding acid and bile tolerance, B. subtilis and L. acidophilus could not survive for 1 h at pH 1.0, whereas, L. plantarum and L. brevis could demonstrate low survival percentages. All strains showed an acid tolerance when incubated at pH 2.0 or 3.0 over a period of 4 h. The highest survival percentage in the acidic conditions occurred with L. acidophilus. B. subtilis recorded the highest bile tolerance over lactobacilli. The sensitivity response of probiotic as well as indicator strains to antibiotics varied.
 [Fulltext PDF]   [References]   [View Citation]  [Report Citation]
 RELATED ARTICLES:
  •    Effect of Probiotics on the Haematological Parameters of Indian Magur (Clarius batrachus L.)
  •    Probiotics: The New Ecofriendly Alternative Measures of Disease Control for Sustainable Aquaculture
How to cite this article:

Moustafa Y.M. El-Naggar , 2004. Comparative Study of Probiotic Cultures to Control the Growth of Escherichia coli O157: H7 and Salmonella typhimurium. Biotechnology, 3: 173-180.

DOI: 10.3923/biotech.2004.173.180

URL: https://scialert.net/abstract/?doi=biotech.2004.173.180

 
COMMENT ON THIS PAPER
.
 
 
 
 

 

 
 
 
 
 
 
 
 
 

 
 
 
 
 
 
 
 

Curve Bottom